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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture Scientia Agricola
Pompeu,Georgia Bertoni; Gratão,Priscila Lupino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
Exposure to nickel (Ni) at high concentrations can lead to production of reactive oxygen species (ROS) resulting in oxidative damage at the cellular level. We investigated the antioxidative responses of Nicotiana tabacum cv BY-2 cell suspension to Ni stress (0.075 and 0.75 mM NiCl2) over a 72 h period with special attention to potential alterations in isoenzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR). Two main SOD isoenzymes were observed, a Mn-SOD (band I) and a Fe-SOD (band II), as well as one CAT isoenzyme and four GR isoenzymes. Activity staining analysis revealed that CAT activity plays a major role in the early response to Ni-induced oxidative stress, particularly when the Ni concentration used was low, whilst a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Catalase; Nickel; Superoxide dismutase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500015
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Fotossíntese, remobilização de reservas e crescimento de grãos em dois híbridos de milho sob deficiência hídrica na fase de enchimento dos grãos Bragantia
Machado,Eduardo Caruso; Silveira,Joaquim Albenísio Gomes da; Vitorello,Victor Alexandre; Rodrigues,Jorge Luiz Mazza.
Foram avaliados, em dois híbridos simples de milho (Zea mays L.), Pioneer 3072 e IAC 701 x B, submetidos à deficiência hídrica, o comportamento da fotossíntese, da remobilização de N e de açúcares durante o período de crescimento dos grãos. As plantas foram cultivadas sob condições naturais, em doze tanques de alvenaria (4 m de comprimento x 0,5 m de largura x 0,6 m de profundidade) contendo terra. A deficiência hídrica foi induzida entre o 5.º e o 15.º dia após a floração, suspendendo-se a irrigação. Sem deficiência hídrica, ambos os híbridos apresentaram comportamento semelhante quanto aos parâmetros avaliados. Sob deficiência hídrica, a remobilização de açúcares e de N, a partir dos órgãos vegetativos para os grãos, foi mais intensa que nas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milho; Zea mays L.; Deficiência hídrica; Trocas gasosas; Crescimento; Açúcares; Nitrogênio.
Ano: 1992 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051992000200004
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Recent advances in aluminum toxicity and resistance in higher plants Braz. J. Plant Physiol.
Vitorello,Victor Alexandre; Capaldi,Flávia Regina; Stefanuto,Vanderlei Antonio.
Aluminum toxicity is a major soil constraint to food and biomass production throughout the world. Considerable advances in the understanding of the mechanism of resistance involving exudation of organic acids have been made in recent years. However, despite intense research efforts, there are many aspects of Al toxicity that remain unclear. This article reviews the features of the chemistry of Al relevant to its toxicity followed by an examination of the mechanisms of toxicity and resistance. Emphasis, however, is given to the mechanisms of Al toxicity, since resistance has been covered recently by several reviews. Some topics which are specifically discussed in this review are: a) The possible role of cellular effects of low pH in Al toxicity, which has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Al uptake and localization; Cell wall; Cytoskeleton; Differential sensitivity to Al; Low pH; Acidity; Plasma membrane.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100011
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Seed phytate content and phosphorus uptake and distribution in dry bean genotypes Braz. J. Plant Physiol.
Coelho,Cileide Maria Medeiros; Santos,Júlio Cesar Pires; Tsai,Siu Mui; Vitorello,Victor Alexandre.
Phytic acid is the major storage form of phosphorus in seeds of legumes and cereals. Since phytate can form complexes with proteins and minerals, reducing the digestive availability of these nutrients, it is usually regarded as an antinutrient, although recent work indicates that it has important beneficial roles as an antioxidant and anticarcinogen. Therefore, there is an interest in the assessment and manipulation of phytate contents in important food grains such as beans. The objective of this work was to compare eleven dry bean genotypes with regard to grain contents of P, phytate, inorganic P and protein and to examine if differences between genotypes could be explained by differences in grain protein content or in uptake and partitioning of P. Plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inorganic phosphorus; Phaseolus; Phytic acid; Protein.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100007
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Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants BABT
Coelho,Cileide Maria Medeiros; Bellato,Cláudia Mattos; Garcia,Andréia Karime Marcelino; Vitorello,Victor Alexandre; Azevedo,Ricardo Antunes.
The in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). Variation in phytate coincided with different concentrations of sucrose, myo-inositol, P and ABA for the duration tested. These compounds caused an accumulation of phytate and were more effective in the presence of myo-inositol and P. The accumulation of P varied less than phytate for the different treatments tested in vitro. In conclusion, P, sucrose, ABA, and myo-inositol caused an increase in the phytate of bean seed, showing that it could be possible to alter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abscisic acid; Glutamine; Phosphorus; Phytic acid; Sucrose.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100020
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